Generation & Development of Generic HCP CHO Kits

Monitoring host cell proteins during bioprocess development and biologics manufacturing is a critical task often performed using ELISA. Custom process-specific HCP ELISA are typically the preferred method for measuring HCPs; however, it is not always appropriate or practical to generate antibodies and a customized HCP-ELISA. This creates a strong need for robust, ready-to-use, generic HCP assays to support biologics development.

Presented by:
David Chimento, PhD | Vice President of Research and Development, Rockland

Contributions by:
Joe Hirano | Program Manager, Cytiva
Rikki Lund, PhD | Head of Impurity Analysis, Alphalyse

Topics covered

1. Creation
of the mock HCCF as the HCP reference standard

2. Development
of a broad-coverage CHO-HCP antibody

3. Characterization
using orthogonal methods for HCP coverage

Amersham™ CHO HCP Kits

In collaboration with Cytiva Life Sciences (formally, GE Heathcare Life Sciences), Rockland has developed and extensively validated products to provide industry professionals a complete solution that supports Host Cell Protein (HCP) risk mitigation.

© 2022 Rockland Immunochemicals, Inc.